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Most amplifiers used for Wireless ISP purposes (including 2.4Ghz flavors) are rated for output power. Therefore, input power and losses prior to the amplifier can be ignored. Typically, the amplifier is mounted very close to the external amplifier, without any extension cables or extra connectors.
Example 1: PC Card with 0.5 Watt amplifier and 8 dBi external antenna.
Note, 0.5 Watt = 27 dBm.
+ amplfier output + 8 dBi antenna = ? Total output power
27 dBm + 8 dBi = 35 dBm
Example 2: PC Card with 1 Watt amplifier and 6 dBi external antenna.
30 dBm + 6 dBi = 36 dBm